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| Registros recuperados : 14 | |
| 3. |  | BALBINOTTE, J.; NICKEL, O.; SILVA, F. N. da; RADAELLI, P.; VANNI, M. F.; FAJARDO, T. V. M. Avaliação de incidência de Peach latent mosaic virus em pessegueiros e nectarineiras das principais regiões produtoras do Rio Grande do Sul. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 31. (Embrapa Uva e Vinho. Documentos, 63). Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 4. | | BALBINOTTE, J.; LOPES, J. R. S.; PONTES, R. G. M. S. de; PRACA, L. B.; ASTUA, J. de F. Movimentação de Bacillus thuringiensis em plantas cítricas e aquisição por Diaphorina citri Kuwayama (HEMIPTERA: PSYLLIDAE). In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal, RN. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. 1 CD-ROM| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | POLETTO, C. M.; SILVA, G. A. da; POLI, J. S.; BALBINOTTE, J.; CARBONERA, A.; BARBOSA, E. dos S.; LOCATELLI, C.; VALENTE, P. Inibição do metabolismo de diferentes linhagens de Saccharomyces cerevisiae induzida por Brettanomyces custersianus. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 6.; ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 2., 2008, Bento Gonçalves. Resumos... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 49. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | SILVA, G. A. da; POLETTO, C. M.; POLI, J. S.; BALBINOTTE, J.; CARBONERA, A. de S.; SOUZA, E. de; LOCATELLI, C.; VALENTE, P. Comportamento de diferentes linhagens de Saccharomyces cerevisiae na presença da levedura Brattanomyces custersianus durante a fermentação tumultuosa da vinificação em tinto. In: CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 12., 2008, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 2008. p. 157. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | DORTA, S. O.; BALBINOTTE, J.; LOPES, J. R. S.; PONTES, R. G. M. S. de; MACHADO, M. A.; ASTUA, J. de F. Pathogenicity of Bacillus thuringiensis to Diaphorina citri Kuwayama (Hemiptera: Liviidae) In: HEMIPTERAN-PLANT INTERACTIONS SYMPOSIUM, 3., 2017, Madri. From sub-cellular hemipteran-host plant-pathogen interactions to pest/disease management: book of abstracts... Madri: ICA-CSIC, 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | DORTA, S. O.; BALBINOTTE, J.; LOPES, J. R. S.; PONTES, R. G. M. S. de; MACHADO, M. A.; ASTUA, J. de F. Pathogenicity of Bacillus thuringiensis to Diaphorina citri Kuwayama (Hemiptera: Liviidae) In: HEMIPTERAN-PLANT INTERACTIONS SYMPOSIUM, 3., 2017, Madri. From sub-cellular hemipteran-host plant-pathogen interactions to pest/disease management: book of abstracts... Madri: ICA-CSIC, 2017.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | ASTUA, J. de F.; MARTINS, P. K.; MAFRA, V. S.; BALBINOTTE, J.; LOPES, J. R. S.; PONTES, R. G. M. S. de; MACHADO, M. A. Uso de ferramentas biotecnológicas para o manejo do HLB dos citros. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | ASTUA, J. de F.; MARTINS, P. K.; MAFRA, V. S.; BALBINOTTE, J.; LOPES, J. R. S.; PONTES, R. G. M. S. de; MACHADO, M. A. Uso de ferramentas biotecnológicas para o manejo do HLB dos citros. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 22., 2012, Bento Gonçalves. Anais... Bento Gonçalves: SBF, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. Insect Science, p.1-12, 2019.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. Insect Science, v. 27, n. 3, p. 519-530, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
19/10/2020 |
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Data da última atualização: |
19/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
DORTA, S. de O.; BALBINOTTE, J.; MONNERAT, R.; LOPES, J. R. S.; CUNHA, T. da; ZANARDI, O. Z.; MIRANDA, M. P. de; MACHADO, M. A.; ASTUA, J. de F. |
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Afiliação: |
SILVIA DE OLIVEIRA DORTA, ESALQ; JULIANA BALBINOTTE, Centro de Citricultura Sylvio Moreira; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen; JOAO ROBERTO SPOTTI LOPES, Centro de Citricultura Sylvio Moreira; TATIANE DA CUNHA, ESALQ; ODIMAR ZANUZO ZANARDI, Fundecitrus; MARCELO PEDREIRA DE MIRANDA, Fundecitrus; MARCOS ANTONIO MACHADO, Centro de Citricultura Sylvio Moreira; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
Selection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera: Liviidae) nymphs. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Insect Science, v. 27, n. 3, p. 519-530, 2020. |
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DOI: |
https://doi.org/10.1111/1744-7917.12654 |
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Idioma: |
Inglês |
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Conteúdo: |
Bacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus. MenosBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for... Mostrar Tudo |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus NAL: |
Citrus; Diaphorina citri; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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Marc: |
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024 7 $ahttps://doi.org/10.1111/1744-7917.12654$2DOI
100 1 $aDORTA, S. de O.
245 $aSelection of Bacillus thuringiensis strains in citrus and their pathogenicity to Diaphorina citri (Hemiptera$bLiviidae) nymphs.$h[electronic resource]
260 $c2020
520 $aBacillus thuringiensis (Bt) toxins are effective in controlling insect pests either through the spraying of products or when expressed in transgenic crops. The discovery of endophytic Bt strains opened new perspectives for studies aimed at the control of sap-sucking insects, such as the Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae), a vector of "Candidatus Liberibacter spp.," associated with citrus huanglongbing (HLB). In this study, translocation of endophytic Bt strains in citrus seedlings inoculated with Bt suspension delivered by soil-drench, and their systemic pathogenicity to D. citri nymphs were investigated. The pathogenicity of three wild-type Bt strains against D. citri third-instar nymphs was demonstrated. Among the 10 recombinant strains tested (each of them harboring a single cry or cyt gene), 3 can be highlighted, causing 42%-77% and 66%-90% nymphal mortality at 2 and 5 d after inoculation, respectively. The isolation of Bt cells from young citrus shoots and dead nymphs, and PCR performed with specific primers, confirmed the involvement of the Bt strains in the psyllid mortality. This is the first report showing the translocation of Bt strains from citrus seedling roots to shoots and their potential to control D. citri nymphs that fed on these soil-drench inoculated seedlings. The Bt strains that caused the highest mortality rates have the potential to be used as bioinsecticides to control D. citri and the identified genes can be used for the production of transgenic Bt citrus.
650 $aCitrus
650 $aDiaphorina citri
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aBALBINOTTE, J.
700 1 $aMONNERAT, R.
700 1 $aLOPES, J. R. S.
700 1 $aCUNHA, T. da
700 1 $aZANARDI, O. Z.
700 1 $aMIRANDA, M. P. de
700 1 $aMACHADO, M. A.
700 1 $aASTUA, J. de F.
773 $tInsect Science$gv. 27, n. 3, p. 519-530, 2020.
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